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    Recombinant Human B7-H3(4Ig)/B7-H3b (hFc Tag)

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    產品信息

     

    產品名稱

    產品編號

    規格

    價格(元)

    Recombinant Human B7-H3(4Ig)/B7-H3b (hFc Tag)

    93111ES25

    25 μg

    1185

    93111ES60

    100 μg

    3185

    93111ES76

    500 μg

    12235

    93111ES80

    1 mg

    21825

     

    產品描述

     

    Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses. Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V-like and Ig C-like domains with a short cytoplasmic tail. Among the family members there is about 20 - 40% amino acid (aa) sequence identity. B7-H3 was initially reported to be a 316 aa type I transmembrane precursor protein that contained a signal sequence, an extracellular region with one V-type and one C-type Ig domain, a transmembrane segment and a short cytoplasmic tail. Subsequent studies have identified a second 110 kDa form whose precursor is 534 aa in length. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V?type and one C-type) and shows a ubiquituous expression pattern. It would appear that the human 4Ig form is the principal, if not the only form of B7-H3. Its precursor contains a 26 aa signal sequence, a 435 aa extracellular region, a 31 aa transmembrane domain, and a 42 aa cytoplasmic tail. The four Ig-like domains alternate between V-type and C?type, and apparently are the consequence of a V-C type tandem duplication. B7-H3b is expressed on dendritic cells as well as activated T, B and NK cells.

     

    產品性質

     

    別名

    B7-H3; CD276; PSEC0249; 4Ig-B7-H3; UNQ309; PRO352; B7 homolog 3; CD276

    Uniprot No.

    Q5ZPR3.1

    表達區間及表達系統

    Recombinant Human B7-H3 (4Ig)/B7-H3b Protein is expressed from HEK293 Cells with hFc tag at the C-terminal. It contains Gly27-Thr461.

    分子量

    Approximately 73.4 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.

    純度

    > 95% as determined by SDS-PAGE and HPLC.

    活性

    ELISA Data: Immobilized Human B7-H3 (4Ig) , hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 44.3ng/ml determined by ELISA.

    內毒素

    1.0 EU per 1μg of the protein by the LAL method.

    制劑

    Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

    復溶

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.


     

    運輸與保存方法

     

    冰袋運輸。-20-80存,一年有效期。

    復溶后,-20 -80°C,在未開封狀態下保存 3-6 個月。復溶后,2-8°C 保存 2-7 天。

    建議第一次使用時分裝存,避免反復凍融。

     

    注意事項

     

    1. 避免反復凍融。

    2. 為了您的安全和健康,請穿實驗服并戴一次性手套操作。

    3. 本產品僅作科研用途!

     

    產品數據


    image.png


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    HB220804


    400-6111-883

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