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產品信息
產品名稱 | 產品編號 | 規格 | 價格（元） |
Recombinant Human B7-H3(4Ig)/B7-H3b (hFc Tag) | 93111ES25 | 25 μg | 1185 |
93111ES60 | 100 μg | 3185 | |
93111ES76 | 500 μg | 12235 | |
93111ES80 | 1 mg | 21825 |
產品描述
Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses. Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V-like and Ig C-like domains with a short cytoplasmic tail. Among the family members there is about 20 - 40% amino acid (aa) sequence identity. B7-H3 was initially reported to be a 316 aa type I transmembrane precursor protein that contained a signal sequence, an extracellular region with one V-type and one C-type Ig domain, a transmembrane segment and a short cytoplasmic tail. Subsequent studies have identified a second 110 kDa form whose precursor is 534 aa in length. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V?type and one C-type) and shows a ubiquituous expression pattern. It would appear that the human 4Ig form is the principal, if not the only form of B7-H3. Its precursor contains a 26 aa signal sequence, a 435 aa extracellular region, a 31 aa transmembrane domain, and a 42 aa cytoplasmic tail. The four Ig-like domains alternate between V-type and C?type, and apparently are the consequence of a V-C type tandem duplication. B7-H3b is expressed on dendritic cells as well as activated T, B and NK cells.
產品性質
別名 | B7-H3; CD276; PSEC0249; 4Ig-B7-H3; UNQ309; PRO352; B7 homolog 3; CD276 |
Uniprot No. | Q5ZPR3.1 |
表達區間及表達系統 | Recombinant Human B7-H3 (4Ig)/B7-H3b Protein is expressed from HEK293 Cells with hFc tag at the C-terminal. It contains Gly27-Thr461. |
分子量 | Approximately 73.4 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result. |
純度 | > 95% as determined by SDS-PAGE and HPLC. |
活性 | ELISA Data: Immobilized Human B7-H3 (4Ig) , hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 44.3ng/ml determined by ELISA. |
內毒素 | < 1.0 EU per 1μg of the protein by the LAL method. |
制劑 | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
復溶 | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
運輸與保存方法
冰袋運輸。-20℃至-80℃保存，一年有效期。
復溶后，-20 至 -80°C，在未開封狀態下保存 3-6 個月。復溶后，2-8°C 保存 2-7 天。
建議第一次使用時分裝凍存，避免反復凍融。
注意事項
1. 避免反復凍融。
2. 為了您的安全和健康，請穿實驗服并戴一次性手套操作。
3. 本產品僅作科研用途！
產品數據
HB220804
400-6111-883
