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    Biotinylated Recombinant Human MSLN/Mesothelin (His-Avi Tag)

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    產品信息

     

    產品名稱

    產品編號

    規格

    價格(元)

    Biotinylated Recombinant Human MSLN/Mesothelin (His-Avi Tag)

    93114ES25

    25 μg

    2245

    93114ES60

    100 μg

    6225

    93114ES76

    500 μg

    21985

    93114ES80

    1 mg

    39985

     

    產品描述

     

    Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation. This recombinant human Mesothelin lacks the 8 aa insertion, and within aa 296-580 it shares 59% sequence identity with mouse and rat Mesothelin. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is upregulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression.

     

    產品性質

     

    別名

    MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR

    Uniprot No.

    Q13421.2

    表達區間及表達系統

    Recombinant Biotinylated Human MSLN/Mesothelin Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Glu296-Gly580.

    分子量

    Approximately 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

    純度

    > 95% as determined by SDS-PAGE and HPLC.

    活性

    ELISA Data: Immobilized Biotinylated Human MSLN at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 20.3ng/ml determined by ELISA.

    內毒素

    1.0 EU per 1μg of the protein by the LAL method.

    制劑

    Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

    復溶

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

     

    運輸與保存方法

     

    冰袋運輸。-20-80存,一年有效期。

    復溶后,-20 -80°C,在未開封狀態下保存 3-6 個月。復溶后,2-8°C 保存 2-7 天。

    建議第一次使用時分裝存,避免反復凍融。

     

    注意事項

     

    1. 避免反復凍融。

    2. 為您的安全和健康,請穿實驗服并戴一次性手套操作。

    3. 本產品僅作科研用途!

     

    產品數據


    image.png

    HB220805


    400-6111-883

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