Biotinylated Recombinant Human MSLN/Mesothelin (His-Avi Tag)
Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation. This recombinant human Mesothelin lacks the 8 aa insertion, and within aa 296-580 it shares 59% sequence identity with mouse and rat Mesothelin. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is upregulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression.
MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR
Recombinant Biotinylated Human MSLN/Mesothelin Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Glu296-Gly580.
Approximately 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
> 95% as determined by SDS-PAGE and HPLC.
ELISA Data: Immobilized Biotinylated Human MSLN at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 20.3ng/ml determined by ELISA.
< 1.0 EU per 1μg of the protein by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
復溶后，-20 至 -80°C，在未開封狀態下保存 3-6 個月。復溶后，2-8°C 保存 2-7 天。