Recombinant Human CD38 (hFc Tag)
CD38, also known as ADP-ribosyl cyclase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)+ into three different products with calcium mobilizing ability, cyclic ADP-ribose, NAADP+, and ADP-ribose. CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells. Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic beta cells.
ADPRC 1; T10; CD38; ADP-ribosyl cyclase 1; cADPr hydrolase 1; CD38 molecule; EC 126.96.36.199; EC 188.8.131.52
Recombinant Human CD38 Protein is expressed from HEK293 Cells with hFc tag at the C-terminal. It contains Val43-Ile300.
Approximately 56.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
> 95% as determined by SDS-PAGE and HPLC.
ELISA Data: Immobilized Human CD38, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CD38 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA.
< 1.0 EU per 1μg of the protein by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
復溶后，-20 至 -80°C，在未開封狀態下保存 3-6 個月。復溶后，2-8°C 保存 2-7 天。