Recombinant Human CD5 (hFc-Avi Tag)
CD5, also known as Leu-1, Ly-1, and T1, is a 67 kDa transmembrane glycoprotein in the scavenger receptor superfamily. CD5 has been shown to interact homophilically, with CD72 on B cells, and with beta-glucan components of fungal cell walls. CD5 expression on developing thymocytes is positively regulated by signaling through the T cell antigen receptor (TCR) and is up?regulated on tolerized peripheral CD4+ cells. It inhibits TCR signaling and promotes T cell nonresponsiveness and survival. CD5 signaling inhibits the generation of regulatory T cells but promotes the development of Th17 cells. Within the B cell lineage, CD5 is expressed on B-1a cells, anergic B cells, and IL-10 producing regulatory B cells. Similarly to on T cells, it negatively regulates signaling through the B cell antigen receptor and supports peripheral B cell survival, anergy, and tolerance. B cells can produce an intracellularly-retained form of CD5 which lacks the signal peptide and a portion of the first SRCR domain. CD5 is also involved in the cellular entry of hepatitis C virus into T cells.
CD5 molecule; CD5; LEU1; T1; CD5 antigen
Recombinant Human CD5 Protein is expressed from HEK293 Cells with hFc tag and Avi tag at the C-terminal. It contains Arg25-Asn371.
Approximately 67.1 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Tris-Bis PAGE result.
> 95% as determined by SDS-PAGE and HPLC.
ELISA Data: Immobilized Human CD5 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD5 Antibody, hFc Tag with the EC50 19.6ng/ml determined by ELISA.
< 1.0 EU per 1μg of the protein by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
復溶后，-20 至 -80°C，在未開封狀態下保存 3-6 個月。復溶后，2-8°C 保存 2-7 天。