產品信息

 

產品名稱	產品編號	規格	價格（元）
Biotinylated Recombinant Human Her3/ErbB3 Protein ,His-Avi Tag	93593ES25	25 μg	2885
	93593ES60	100 μg	6885
	93593ES76	500 μg	24115
	93593ES80	1 mg	39885

 

產品描述

 

ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. The cytoplasmic domain of ErbB3 also contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of phosphoinositide 3-kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85.

 

產品性質

 

別名	ErbB3; ErbB-3; HER3; HER3c-erbB-3; LCCS2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; ERBB3
Uniprot No.	P21860.1
表達區間及表達系統	Recombinant Biotinylated Human Her3/ErbB3 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Ser20-Thr643.
分子量	Approximately 71.6 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.
純度	> 95% as determined by SDS-PAGE and HPLC.
活性	ELISA Data: Immobilized Biotinylated Human Her3 at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-Her3 Antibody, hFc Tag with the EC50 17.6 ng/mLdetermined by ELISA.
內毒素	< 1.0 EU per 1μg of the protein by the LAL method.
制劑	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
復溶	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

運輸與保存方法

 

冰袋運輸。-20℃保存，一年有效期。

復溶后，-20至-80°C，在未開封狀態下保存 3-6 個月。復溶后，2-8°C 保存 2-7 天。

建議第一次使用時分裝凍存，避免反復凍融。

 

注意事項

 

1. 避免反復凍融。

2. 為了您的安全和健康，請穿實驗服并戴一次性手套操作。

3. 本產品僅作科研用途！

 

產品數據

 

 

HB220930